Application Note
Simple Western Size Normalization
Accurate normalization is essential for reliable protein expression analysis. However, traditional loading controls such as GAPDH and β-Actin can vary significantly in tissue samples due to physiological and pathological factors, potentially introducing unwanted variability into your data.
In this application note, our scientists evaluate the performance of the Total Protein Assay (TPA) against commonly used housekeeping proteins using the Jess™ Simple Western platform. The study compares normalization strategies for Akt and phospho-Akt analysis across multiple colon cancer tissue lysates and highlights the impact on assay precision and reproducibility.
