Application Note
Immunohistochemical Detection of Phospho-HER-3 in Human Breast Cancer
Immunohistochemistry (IHC) is a powerful tool for understanding tumor biology, but variability in sample handling and antibody performance can compromise results. Ensuring specificity and accuracy is critical to generate meaningful insights for cancer research and drug development.
This application note outlines a robust, standardized approach to developing high-quality IHC protocols, focusing on the detection of phosphorylated HER3 in human breast cancer tissue. It highlights key considerations such as antibody validation, control selection, and optimization steps to ensure reliable and reproducible staining outcomes.
